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KMID : 0613820020120010055
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Journal of Life Science
2002 Volume.12 No. 1 p.55 ~ p.60
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Effect of trichostatin A on NF-κB DNA binding activity and myogenesis in C2C12 skeletal muscle precursor cell
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Kim Kyung-Chang
Shin Hae-Ja
Lim Woon-Ki
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Abstract
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The differentiation of skeletal muscle precursor cells in culture is marked by the transcriptional activation of muscle-specific genes and the morphological differentiation of myoblast into multinucleate myotube. In this study, we examined the effect of TSA (Trichostatin A) on NF-κB DNA binding activity and muscle cell fusion in the process of myogenesis. Under TSA treatment, C2C12 myoblast could not fuse to myotube and its NF-κB DNA binding activity was also blocked. To investigate whether these phenomenons were affected by TSA in direct or not, differentiation media (DM) used to differentiate cells without TSA was concentrated and added to C2C12 myoblast with TSA simultaneously. C2C12 myoblast was fused to myotube and NF-κB DNA binding activity was recovered. These results suggest that TSA affects on the differentiation of myoblast, perhaps through several factors, by inhibiting myoblst fusion and blocking NF-κB DNA binding activity.
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KEYWORD
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myogenesis, TSA, NF-κB, C2C12 myoblast
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